American Society for Peripheral Nerve

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Evaluation of Neural Tissue Regeneration Mediated by a Functional Peptide, Ln2-P3
Sang Wha Kim, MD, PhD
Department of Plastic and Reconstructive Surgery, Seoul national University hospital, Seoul, Korea, Republic of (South); Youn Hwan Kim, MD, PhD; Department of Plastic and Reconstructive Surgery, Hanyang University, Seoul, Korea, Republic of (South)

Segmental nerve defects pose a daunting clinical challenge, as peripheral nerve injury studies have established that there is a critical nerve gap length for which the distance cannot be successfully bridged with current techniques. Construction of a neural conduit the peptide was effective in promoting cell attachment and spreading in vitro. The object of this study was to examine the effects of Ln2-P3 in peripheral nerve regeneration in segmental nerve defects in a rat sciatic nerve injury model.

To examine the effects of Ln2-P3 in peripheral nerve regeneration in vivo, we collagen tube as an artificial nerve graft. The novel graft was coated with Ln2-P3 and used to bridge a 10?mm defect in rat sciatic nerves. Control groups were treated with serum only-filled conduits of scrambled peptide coated conduits. Animals were assessed for the histopathologic examination in the proximal, middle, and distal portions of the channel, motor function using sciatic functional index, and nerve conduction test.

The addition of peptide to the nerve conduit enhanced the regeneration of myelinated axons from the nerve stump compared a serum-only filled conduit and scrambled peptide conduit. Also the SFI revealed a significant improvement in functional recovery.

These findings indicate that Ln2-P3, combined with collagen conduit, has potential biomedical applications in peripheral nerve injury repair.

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